Western Blot analysis
The Western Blot analysis is a biochemical tecnique that permits the identification of a particular protein among a solution, using antibodies able to recognize and bind it specifically. Its detection is obtained through the analysis of the signal generated from the enzyme bound to the antibody used for the protein of interest. The recorded signal intensity is directly proportional to the amount of the bound protein, allowing the evaluation of the possible protein levels variation in different conditions.
The enzyme are proteins with catalitic activity, able to perform several kind of reactions. It is therefore important that industrially synthesized enzymes conduct their work properly. We are able to measure the enzymatic activity through specific assays, evaluating the substrate consumption or the product synthesis, by colorimetric and/or spectrophotometric analyses for example.
Who it is for: companies, research institutions.